ANO1 Knockout cell line (PC-3)

Loci	STR Info (Sample Cell) Sample Cell Line:PC-3			STR Info (Cell bank) Cell Line:PC-3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	13	13		13	13
D13S317	11	11		11	11
D7S820	8	11		8	11
D16S539	11	11		11	11
VWA	17	17		17	17
TH01	6	7		6	7
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	11	11		11	11
D12S391	21	21
FGA	24	24
D2S1338	18	20
D21S11	29	31.2
D18S51	14	15
D8S1179	13	13
D3S1358	16	16
D6S1043	18	18
PENTAE	10	17
D19S433	14	17.2
PENTAD	9	9
D1S1656	12	16

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ANO1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PC-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ANO1 gene knockout ANO1 Knockout cell line（PC-3） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ANO1 Knockout cell line (PC-3)

Catalog#

YKO-H1160

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Prostate Carcinoma Cell Line(PC-3)
inquiry More details

Catalog#: YC-C010

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

Ano1 Knockout cell line (Raw 264.7)
inquiry More details

Organism: Mouse

Gene Name: Ano1

Gene ID: 101772

Size: 1*10^6

Catalog#: YKO-MT0045

YKO-MT0045(Raw 264.7)
ANO1 Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: ANO1

Gene ID: 55107

Size: 1*10^6

Catalog#: YKO-HS13463

YKO-HS13463(HCT 116)
ANO1 Knockout cell line (A549)
inquiry More details

Organism: Human

Gene Name: ANO1

Gene ID: 55107

Size: 1*10^6

Catalog#: YKO-HS13461

YKO-HS13461(A549)

Related Popular Products

Wild-type Cell Lines

Human Prostate Carcinoma Cell Line(PC-3)

Price (USD): -

Catalog#: YC-C010

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

Ano1 Knockout cell line (Raw 264.7)

Organism: Mouse

Gene Name: Ano1

Gene ID: 101772

Size: 1*10^6

Catalog#: YKO-MT0045

inquiry More details
ANO1 Knockout cell line (HCT 116)

Organism: Human

Gene Name: ANO1

Gene ID: 55107

Size: 1*10^6

Catalog#: YKO-HS13463

inquiry More details
ANO1 Knockout cell line (A549)

Organism: Human

Gene Name: ANO1

Gene ID: 55107

Size: 1*10^6

Catalog#: YKO-HS13461

inquiry More details

ANO1 Knockout cell line (PC-3)

Subscribe Us